NGenePlex nCoV/FluA.B Detection Kit

NGenePlex nCoV/FluA.B Detection Kit


The NGenePlex ™ nCoV qRT-PCR Kit is a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay reagent designed for the qualitative detection of SARS-CoV-2 nucleic acid from infected or suspected patients of COVID-19.

  • The NGenePlex ™ nCoV qRT-PCR Kit is designed for use by qualified laboratory personnel specifically trained and instructed in qRT-PCR techniques as an in vitro diagnostic kit.
  • In addition, the intended use is to detect SARS-CoV-2 nucleic acid in infected or suspected COVID-19 patients with symptoms including fever, cough, fatigue, shortness of breath, and loss of smell and taste.
  • Samples can be collected from a respiratory tract, such as a nasal, nasopharyngeal (PN), or oropharyngeal (OP) swab.


  • Certification: CE-IVD
  • Number: 96 tests
  • Target genes: RdRp and N genes in SARS-CoV-2
  • Sample types: human nasopharyngeal swab (PN), oropharyngeal swab (OP)

Validated Instruments:

  • * CFX96 ™ Real-Time PCR Detection System (Bio-Rad)
  • ABI 7500 Real-Time PCR System (ThermoFisher Scientific)
  • QuantStudio 3 Real-Time PCR Instrument (ThermoFisher Scientific)
  • LightCycler® 480 System (Roche)

Fluorometer channel:

  • FAM (RdRp)
  • HEX / VIC (N)
  • Cal Red 610 / Texas Red (IC: Human RNase P)

The primers and probes of the NGenePlex ™ nCoV qRT-PCR kit were designed to target the RdRP1 gene in an open reading frame (orf1ab) that encodes non-structural proteins (nsp) for replication and the N gene that encodes the nucleocapsid protein. In addition, an additional primer-probe set is included in the kit to detect the human RNase P gene as an internal control (IC).

This kit contains a ready-to-use, one-step system for the detection of 2019-nCoV by reverse transcription-polymerase chain reaction (RT-PCR) in the real-time PCR system. The reagent mix contains target sequences and target probes for specific amplification of virus RNA. The reaction is performed in a real-time RT-PCR step, which includes the following;

  • A reverse transcription (RT), where the RNA of the virus is transcribed into cDNA.
  • Amplification (PCR), where a thermostable DNA polymerase is used to amplify specific gene fragments.
  • Quantification, where the fluorescence is emitted and measured by the optical unit of the systems in real-time during the PCR.

1. Viral growth/titration

Viral titration assays allow for the quantification of virus concentration and the production and isolation of the purified virus. BioTek imaging and microscopy instruments with Gen5 image analysis software automate viral growth and titration assays.

  • CPE detection
  • TCID50 calculation
  • Fluorescent FFA
  • plate test

2. Viral replication kinetics

The kinetics of viral growth and replication provide key information for understanding virus-host interactions. BioTek Imaging and Microscopy instruments and automation accessories provide live-cell imaging and analysis capabilities.

  • Viral growth kinetics
  • Subcellular localization during the virus life cycle.
  • replication complexes

3. Viral detection

For both basic research and clinical applications, the ability to rapidly detect the virus in a sample is key to any virology project. With BioTek detection and imaging instruments, viruses can be detected by a variety of methods, while BioTek liquid handlers and stackers increase assay efficiency and throughput.

  • Fast ELISA Screen
  • Fluorescent detection of immunolabeled/reporter strains
  • Antibody screening for viral detection