NFKB2 – Ab-866 Polyclonal Antibody

The NFKB2 – Ab-866 Polyclonal Antibody is a highly specific and versatile antibody designed for detecting NF-kappa B2 (NFKB2) protein, a critical transcription factor involved in regulating immune response, inflammation, apoptosis, and cell proliferation. This antibody is particularly useful for studying the canonical and non-canonical NF-kappa B signaling pathways, which are implicated in various physiological and pathological processes such as cancer, autoimmune diseases, and chronic inflammatory conditions.

Background on NFKB2

NFKB2 is a member of the NF-kappa B/Rel protein family and exists in two main forms:

• p100: An inactive precursor that undergoes proteasomal processing to generate p52.
• p52: An active transcription factor that forms heterodimers with RelB to regulate gene expression.

NFKB2 is involved in:

1. Immune Response: Regulating cytokine production and immune cell activation.
2. Lymphoid Organogenesis: Critical for the formation of secondary lymphoid organs.
3. Cancer Progression: Aberrant activation of NFKB2 is associated with tumorigenesis and resistance to therapy.
4. Chronic Inflammation: Sustained NFKB2 activation contributes to conditions like rheumatoid arthritis and atherosclerosis.

For more detailed information on NFKB2 function, refer to NIH resources and PubMed.

Key Features of NFKB2 – Ab-866 Polyclonal Antibody

1. High Specificity: Targets both p100 and p52 forms of NFKB2 with minimal cross-reactivity.
2. Polyclonal Production: Recognizes multiple epitopes, enhancing sensitivity and robustness.
3. Broad Application: Validated for Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and ELISA.
4. High Purity: Affinity-purified to ensure minimal background noise in experiments.

Applications

1. Western Blot (WB)

• Detect and differentiate NFKB2 isoforms in cell lysates or tissue extracts.
• Applications: Studies on NFKB2 processing in cancer, immune cell signaling, and inflammatory responses.

2. Immunohistochemistry (IHC)

• Visualize NFKB2 distribution in tissue sections.
• Applications: Assessing NFKB2 expression in diseased tissues like tumor biopsies or inflamed organs.

3. Immunofluorescence (IF)

• Localize NFKB2 within cells using fluorescently labeled secondary antibodies.
• Applications: Visualizing nuclear translocation of NFKB2 during activation.

4. Chromatin Immunoprecipitation (ChIP)

• Investigate NFKB2 binding to DNA regulatory regions to understand its role in gene expression.
• Applications: Decoding transcriptional networks regulated by NFKB2.

5. Enzyme-Linked Immunosorbent Assay (ELISA)

• Quantify NFKB2 levels in cell lysates or biological fluids.
• Applications: Monitoring NFKB2 activity under different experimental conditions.

Specifications

Storage and Stability

• Storage Conditions: Store at -20°C for long-term use. For frequent use, aliquot to avoid freeze-thaw cycles.
• Stability: Guaranteed stable for 12 months when stored under recommended conditions.

Recommended Protocols

1. Western Blot

1. Prepare protein samples (20-30 µg per lane).
2. Perform SDS-PAGE and transfer proteins to a PVDF or nitrocellulose membrane.
3. Block membrane with 5% BSA in TBST for 1 hour.
4. Incubate with NFKB2 – Ab-866 Antibody (1:1,000 dilution) overnight at 4°C.
5. Wash and incubate with HRP-conjugated secondary antibody (1:10,000 dilution) for 1 hour.
6. Visualize bands using ECL substrate.

2. Immunohistochemistry

1. Deparaffinize and rehydrate tissue sections.
2. Perform antigen retrieval in citrate buffer (pH 6.0) at 95°C for 15 minutes.
3. Block with 5% normal serum for 30 minutes.
4. Incubate with NFKB2 – Ab-866 Antibody (1:200 dilution) for 1 hour at room temperature.
5. Apply secondary antibody and develop with DAB chromogen.

3. Immunofluorescence

1. Fix cells with 4% paraformaldehyde for 15 minutes.
2. Permeabilize with 0.1% Triton X-100 for 10 minutes.
3. Block with 3% BSA for 30 minutes.
4. Incubate with NFKB2 – Ab-866 Antibody (1:500 dilution) for 1 hour.
5. Use fluorescently labeled secondary antibodies and counterstain with DAPI.

Research Applications

1. Cancer Biology: Studying the role of NFKB2 in tumor progression, metastasis, and resistance to therapy.
2. Autoimmune Diseases: Investigating NFKB2 dysregulation in conditions like lupus and multiple sclerosis.
3. Chronic Inflammation: Understanding how NFKB2 drives cytokine production in diseases like Crohn’s and rheumatoid arthritis.
4. Viral Pathogenesis: Exploring NFKB2’s involvement in viral latency and immune evasion.

Safety Notes

• This product contains sodium azide as a preservative. Avoid ingestion and skin contact. Dispose of waste according to local regulations.

References and Further Reading

• NIH Research Resources
• PubMed: NFKB2 in Cancer
• KEGG Pathway Database

For additional details, please feel free to reach out or request diagrams and images to complement your research materials!